{"id":3,"date":"2020-05-21T10:38:50","date_gmt":"2020-05-21T10:38:50","guid":{"rendered":"http:\/\/localhost\/blog\/?page_id=3"},"modified":"2020-12-04T14:20:28","modified_gmt":"2020-12-04T14:20:28","slug":"research","status":"publish","type":"page","link":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/?page_id=3","title":{"rendered":"\u696d\u7e3e(Results)"},"content":{"rendered":"\n
Research grants (2010~)<\/h5>\n\n\n\n

\u57fa\u76e4\u7814\u7a76(A)\u3000\u300c\u65b0\u898f\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u958b\u767a\u3092\u898b\u636e\u3048\u305f\u65b0\u898fCas\u30bf\u30f3\u30d1\u30af\u8cea\u5019\u88dc\u306e\u6a5f\u80fd\u89e3\u6790\u300d<\/a>\u5206\u62c5 2020-2022\u5e74(\u4e88\u5b9a)<\/p>\n\n\n\n

\u57fa\u76e4\u7814\u7a76(B)\u3000\u300c\u5929\u7136\u5909\u6027\u30d2\u30b9\u30c8\u30f3\u69d8\u86cb\u767d\u8cea\u306b\u3088\u308b\u3001\u7d50\u6838\u83cc\u306e\u500b\u6027\u306e\u5275\u51fa\u3068\u591a\u69d8\u6027\u7372\u5f97\u306e\u5206\u5b50\u6a5f\u69cb\u300d<\/a>\u5206\u62c5\u30002020-2023\u5e74(\u4e88\u5b9a) <\/p>\n\n\n\n

\u57fa\u76e4\u7814\u7a76(B)\u3000\u300c\u8d85\u5206\u5b50\u30b0\u30e9\u30d5\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308bGWAS\u89e3\u6790\u306e\u7814\u7a76\u300d<\/a> \u4ee3\u8868 \u5e73\u621029-32\u5e74\u5ea6(\u4e88\u5b9a)<\/p>\n\n\n\n

\u5275\u85ac\u7b49\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u7814\u7a76\u652f\u63f4\u57fa\u76e4\u4e8b\u696d(BINDS)\u300c\u5275\u85ac\u7b49\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u7814\u7a76\u3092\u4fc3\u9032\u3059\u308b\u7814\u7a76\u652f\u63f4\u3068\u30c7\u30fc\u30bf\u30b5\u30a4\u30a8\u30f3\u30b9\u300d<\/a> \u5206\u62c5(\u5206\u62c5\u6a5f\u95a2\u4ee3\u8868) \u5e73\u621029\uff5e34\u5e74\u5ea6(\u4e88\u5b9a)<\/p>\n\n\n\n

\u5275\u85ac\u7b49\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u7814\u7a76\u652f\u63f4\u57fa\u76e4\u4e8b\u696d(BINDS)\u300c\u751f\u85ac\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306e\u9ad8\u5ea6\u5316\u3068\u69cb\u9020\u5275\u85ac\u3078\u306e\u5fdc\u7528\u300d<\/a> \u5206\u62c5 \u5e73\u621029\uff5e34\u5e74\u5ea6(\u4e88\u5b9a)<\/p>\n\n\n\n

\u57fa\u76e4\u7814\u7a76(C)\u300c\u30af\u30e9\u30f3\u30d7\u30ed\u30fc\u30c0\u30fcRFC\u306b\u3088\u308b\u30af\u30e9\u30f3\u30d7PCNA\u88c5\u3066\u3093\u6a5f\u69cb\u306e\u69cb\u9020\u751f\u7269\u5b66\u7684\u7814\u7a76\u300d<\/a>\u5206\u62c5 \u5e73\u621026-28\u5e74<\/p>\n\n\n\n

\u57fa\u76e4\u7814\u7a76(B)(2) \u300c\u751f\u4f53\u5206\u5b50\u69cb\u9020\u30c7\u30fc\u30bf\u306e\u30b0\u30e9\u30d5\u306b\u3088\u308b\u7d71\u4e00\u306e\u8a66\u307f\u300d<\/a>\u4ee3\u8868 \u5e73\u621025-27\u5e74\u5ea6<\/p>\n\n\n\n

\u57fa\u76e4\u7814\u7a76(B)(2) \u300c\u30af\u30e9\u30f3\u30d7\u306b\u3088\u308b\u30ec\u30d7\u30ea\u30bd\u30fc\u30e0\u306e\u5236\u5fa1\u6a5f\u69cb\u306e\u6a5f\u80fd\u69cb\u9020\u9023\u95a2\u306e\u7814\u7a76\u300d<\/a>\u5206\u62c5 \u5e73\u621024-27\u5e74<\/p>\n\n\n\n

\u6587\u90e8\u79d1\u5b66\u7701\u3000\u5275\u85ac\u7b49\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u7814\u7a76\u652f\u63f4\u57fa\u76e4\u4e8b\u696d(PDIS)\u300c\u8d85\u5206\u5b50\u30e2\u30c7\u30ea\u30f3\u30b0\u30d1\u30a4\u30d7\u30e9\u30a4\u30f3\u306e\u69cb\u7bc9\u300d<\/a>\u4ee3\u8868 \u5e73\u621024-28\u5e74\u5ea6<\/p>\n\n\n\n

JST \u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u63a8\u9032\u4e8b\u696d(BIRD)\u300c\u5b9f\u8df5\u306b\u3088\u308b\u8d85\u5206\u5b50\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30e2\u30c7\u30ea\u30f3\u30b0\u30b7\u30b9\u30c6\u30e0\u306e\u958b\u767a\u300d<\/a> \u4ee3\u8868 \u5e73\u621020-22\u5e74\u5ea6<\/p>\n\n\n\n

Published Papers, Books, and Patents (2010~)<\/h5>\n\n\n\n

2020<\/p>\n\n\n\n

Oba Y*, <\/em>Konishi K, Yano H, Shibata H, Kato D, Shirai T*. Resurrecting the ancient glow of the fireflies, Science Adv., 6, eabc5705 (2020) DOI: 10.1126\/sciadv.abc5705<\/a><\/p>\n\n\n\n

Mayanagi K*, Oki K, Miyazaki N, Ishino S, Yamagami T, Morikawa K, Kohda D, Shirai T*, Ishino Y*. Two conformations of DNA polymerase D-PCNA-DNA, an archaeal replisome complex, revealed by cryo-electron microscopy, BMC Biol., 18, 152 (2020) doi: 10.1186\/s12915-020-00889-y<\/a><\/p>\n\n\n\n

Hijikata A, Shionyu-Mitsuyama C, Nakae S, Shionyu M, Ota M, Kanaya S, Shirai T*. Knowledge\u2010based structural models of SARS\u2010CoV\u20102 proteins and their complexes with potential drugs, FEBS Lett., (2020) doi: 10.1002\/1873-3468.13806<\/a><\/p>\n\n\n\n

Shirai T*, Terada T. Overview of the big data bioinformatics symposium (2SCA) at BSJ2019, Biophysical Reviews, (2020) DOI 10.1007\/s12551-020-00639-y<\/a><\/p>\n\n\n\n

\u300c\u8010\u71b1\u6027\u30df\u30b9\u30de\u30c3\u30c1\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u5909\u7570\u4f53\u300d, \u767a\u660e\u8005: \u677e\u672c\u3000\u88d5\u4e4b, \u4e0a\u68ee\u9686\u53f8, \u767d\u4e95\u3000\u525b, \u77f3\u91ce\u826f\u7d14, \u76f8\u826f\u6b66\u5b8f, \u77f3\u91ce\u5712\u5b50, \u7279\u98582020-048795 \u4ee4\u548c2\u5e743\u670819\u65e5<\/p>\n\n\n\n

2019<\/p>\n\n\n\n

Nakamae I, Morimoto T, Shima H, Shionyu M, Fujiki H, Yoneda-Kato N, Yokoyama T, Kanaya S, Kakiuchi K, Shirai T, Meiyanto E, Kato JY. Curcumin Derivatives Verify the Essentiality of ROS Upregulation in Tumor Suppression, Molecules, 24, 4067 (2019)<\/a><\/p>\n\n\n\n

Lestari B, Nakamae I, Yoneda-Kato N, Morimoto T, Kanaya S, Yokoyama T, Shionyu M, Shirai T, Meiyanto E, Kato JY. Pentagamavunon-1 (PGV-1) inhibits ROS metabolic enzymes and suppresses tumor cell growth by inducing M phase (prometaphase) arrest and cell senescence, Sci. Rep. 9, 14867 (2019)<\/a><\/p>\n\n\n\n

Nakae S, Shionyu M, Ogawa T, Shirai T*, Crystallization of pearl biomineralization protein in microgravity environment. Int. J. Microgravity Sci. Appl., 36, 2019p360105 (2019) <\/a><\/p>\n\n\n\n

Kouyama K, Mayanagi K, Nakae S, Nishi Y, Miwa M, Shirai, T*., Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1, Biophysics and Physicobiology, 16, 59-67 (2019) <\/a><\/p>\n\n\n\n

Sutani A, Shima H, Hijikata A, Hosokawa S, Fukui Y, Takasawa K, Suzuki E, Doi S, Shirai T, Morio T, Fukami M\u2060, Kashimada K, WDR11 is another causative gene for coloboma, cardiac anomaly and growth retardation in 10q26 deletion syndrome, Eur J Med Genet  (2019)<\/a><\/p>\n\n\n\n

Takasawa K, Tsuji-Hosokawa A, Takishima S, Wada Y, Nagasaki K, Dateki S, Numakura C, Hijikata A, Shirai T, Kashimada K, Morio T, Clinical characteristics of adolescent cases with Type A insulin resistance syndrome caused by heterozygous mutations in the \u03b2-subunit of the insulin receptor (INSR) gene, J. Diabete, 11, 46-54 (2019) <\/a><\/p>\n\n\n\n

\u571f\u65b9\u6566\u53f8, \u8fbb \u654f\u4e4b, \u5869\u751f\u771f\u53f2, \u767d\u4e95 \u525b, \u8d85\u5206\u5b50\u30b0\u30e9\u30d5\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308bGWAS\u89e3\u6790\u306e\u7814\u7a76, \u7d30\u80de, 51, 34 – 38\uff082019\uff09<\/a><\/p>\n\n\n\n

2018<\/p>\n\n\n\n

Isogai Y, Imamura, H, Nakae S, Sumi T, Takahashi K, Nakagawa T, Tsuneshige A, Shirai T*, Tracing whale myoglobin evolution by resurrecting ancient proteins, Sci. Rep. 8, 16883 (2018)<\/a> <\/p>\n\n\n\n

Nishikino T , Hijikata A, Miyanoiri Y, Onoue Y, Kojima S, Shirai T, Homma M, Rotational direction of flagellar motor from the conformation of FliG middle domain in marine Vibrio, Sci. Rep. 8, 17793 (2018) <\/a> <\/p>\n\n\n\n

Mayanagi K, Ishino S, Shirai T, Oyama T, Kiyonari S, Kohda D, Morikawa K, Ishino Y, Direct visualization of DNA baton pass between replication factors bound to PCNA, Sci. Rep. 8, 16209 (2018) <\/a> <\/p>\n\n\n\n

Nakae S, Shionyu M, Ogawa T, Shirai T*, Structures of jacalin\u2010related lectin PPL3 regulating pearl shell biomineralization, Proteins, 86, 644-653 (2018)<\/a><\/p>\n\n\n\n

Shirai T. Protein structure analysis and validation, The Encyclopedia of Bioinformatics and Computational Biology (eds..Shoba Ranganathan, Christian Sch\u00f6nbach, Kenta Nakai, and Michael Gribskov), Elsevier, pp. 512-519 (2018) ISBN: 9780128114148, DOI:10.1016\/B978-0-12-809633-8.20282-3<\/a><\/p>\n\n\n\n

\u6e21\u9089\u535a\u6587\uff0c\u9234\u6728\u6d0b\u4ecb\uff0c\u8fd1\u85e4\u6d0b\u9686\uff0c\u77f3\u91ce\u9ebb\u7531\u5b50\uff0c\u571f\u4e95\u967d\u5b50\uff0c\u6c5f\u53e3\u81f3\u6d0b\uff0c\u7530\u4e2d\u6210\u5178\uff0c\u9db4\u7530\u5b8f\u6a39\uff0c\u767d\u4e95\u3000\u525b\uff0c\u68ee\u4e00\u90ce\uff0c\u81fc\u4e95\u82f1\u4e4b\uff0c\u6a2a\u5ddd\u4e09\u6d25\u592b\uff0c\u9060\u9694\u8b1b\u7fa9\u300c\u8a08\u7b97\u751f\u547d\u79d1\u5b66\u306e\u57fa\u790e\u300d\u914d\u4fe1\u306e\u5b9f\u73fe\uff0c\u5927\u5b66ICT\u63a8\u9032\u5354\u8b70\u4f1a2017\u5e74\u5ea6\u5e74\u6b21\u5927\u4f1a\u8ad6\u6587\u96c6 1 – 4 (2018)<\/p>\n\n\n\n

2017<\/p>\n\n\n\n

Hijikata A, Tsuji T, Shionyu M, Shirai T*, Decoding disease-causing mechanisms of missense mutations from supramolecular structures, Sci. Rep. 7, 8541 (2017) <\/a> <\/p>\n\n\n\n

Miyanori Y, Hijikata A, Nishino Y, Gohara M, Onoue Y, Kojima S, Kojima C, Shirai T, Kainosho M, Homma M, Structural and Functional Analysis of the C-Terminal Region of FliG, an Essential Motor Component of Vibrio Na+-Driven Flagella, Structure, 25, 1540-1548 (2017) <\/a> <\/p>\n\n\n\n

Fujiki R, Hijikata A, Shirai T, Okada S, Kobayashi M, Ohara O, Molecular Mechanism and Structural Basis of Gain of Function of STAT1 Caused by Pathogenic R274Q Mutation, J Biol Chem, 292, 6240-6254 (2017) <\/a> <\/p>\n\n\n\n

Tsuji-Hosokawa A, Takasawa K, Nomura R, Miyakawa Y, Numakura C, Hijikata A, Shirai T, Ogawa Y, Kashimada K, Morio T, Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases, Pediatr Diabetes, 18, 917-924 (2017) <\/a> <\/p>\n\n\n\n

Yoda T, Tanabe M, Tsuji T, Yoda T, Ishino S, Shirai T, Ishino Y, Takeyama H, Nishida H, Exonuclease processivity of archaeal replicative DNA polymerase in association with PCNA is expedited by mismatches in DNA, Sci. Rep. 7, 44582 (2017) <\/a> <\/p>\n\n\n\n

Nomura K, Tanimoto Y, Hayashi F, Harada E, Shan X-Y, Shionyu M, Hijikata A, Shirai T, Morigaki K, Shimamoto K, The Role of the Prod1 Membrane Anchor in Newt Limb Regeneration. Angewandte Chemie, 55, 270-274 (2017)<\/a> <\/p>\n\n\n\n

Satoh Y, Yonemori S, Hirose M, Shogaki H, Wakimoto H, Kitagawa Y, Gotoh B, Shirai T, Takahashi K, Itoh M, A residue located at the junction of the head and stalk regions of measles virus fusion protein regulates membrane fusion by controlling conformational stability. J. Gen. Virol. 98, 143-154 (2017)<\/a> <\/p>\n\n\n\n

Kawasaki Y, Oda H, Ito J, Niwa A, Tanaka T, Hijikata A, Seki R, Nagahashi A, Osawa M, Asaka I, Watanabe A, Nishimata S, Shirai T, Kawashima H, Ohara O, Nakahata T, Nishikomori R, Heike T, Saito MK, Identification of a High-Frequency Somatic NLRC4 Mutation as a Cause of Autoinflammation by Pluripotent Cell-Based Phenotype Dissection. Arthritis & Rheumatology, 69, 447-459 (2017) <\/a><\/p>\n\n\n\n

2016<\/p>\n\n\n\n

Shionyu-Mitsuyama C, Hijikata A, Tsuji T, Shirai T*, Classification of ligand molecules in PDB with graph match-based structural superposition. J. Struct. Funct. Gen., 17, 135-146 (2016) <\/a><\/p>\n\n\n\n

Nakae S, Hijikata A, Tsuji T, Yonezawa K, Kouyama K, Mayanagi K, Ishino S, Ishino Y, Shirai T*, Structure of EndoMS-DNA complex as mismatch-restriction endonuclease. Structure, 24, 1960-1971 (2016) <\/a><\/p>\n\n\n\n

Oyama T, Ishino S, Shirai T, Yamagami T, Nagata M, Ogino H, Kusunoki M, Ishino Y, Atomic structure of an archaeal GAN suggests its dual roles as an exonuclease in DNA repair and a CMG component in DNA replication, Nucleic Acid Res., 44, 2977-2986 (2016) <\/a> <\/p>\n\n\n\n

Takekawa N, Terahara N, Kato T, Gohara M, Mayanagi K, Hijikata A, Onoue Y, Kojima S, Shirai T, Namba K, Homma M, The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium, Sci. Rep. 6, 31526 (2016) <\/a><\/p>\n\n\n\n

Ishino S, Oda S, Uemori T, Sagara T, Takatsu N, Yamagami T, Shirai T, Ishino Y, Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea. Nucleic Acid Res. 44, 2977-2986 (2016) (NAR Breakthrough Article)<\/a><\/p>\n\n\n\n

Yamashita S, Hata A, Usui T, Oda H, Hijikata A, Shirai T, Kaneko N, Hata D, Novel AVPR2 mutation causing partial nephrogenic diabetes insipidus in a Japanese family. J. Pediatr. Endocrinol. Metab. 29, 591-596 (2016) <\/a><\/p>\n\n\n\n

2015<\/p>\n\n\n\n

Tsuji T, Yoda T, Shirai T*, Deciphering Supramolecular Structures with Protein-Protein Interaction Network Modeling. Sci. Rep. 5, 16341 (2015)<\/a> <\/p>\n\n\n\n

Kobayashi A, Teruya K, Mtsuura Y, Shirai T, Nakamura Y, Yamada M, Mizusawa H, Mohri S, Kitamoto T. The influence of PRNP polymorphisms on human prion disease susceptibility: an update. Acta Neuropathol.130, 159-170 (2015) <\/a><\/p>\n\n\n\n

\u571f\u65b9\u6566\u53f8\u3001\u8fbb\u3000 \u654f\u4e4b\u3001\u767d\u4e95\u3000\u525b*, \u8d85\u5206\u5b50\u30e2\u30c7\u30ea\u30f3\u30b0\u306b\u3088\u308b\u5275\u85ac\u652f\u63f4, SAR News, 28, 1-7 (2015)<\/p>\n\n\n\n

\u65e5\u672c\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u5b66\u7de8, \u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u5165\u9580, \u6176\u61c9\u7fa9\u587e\u5927\u5b66\u51fa\u7248\u4f1a (2015)<\/a><\/p>\n\n\n\n

\u767d\u4e95\u3000\u525b, \u30bf\u30f3\u30d1\u30af\u8cea\u7acb\u4f53\u69cb\u9020\u6563\u6b69\uff1a\u7956\u5148\u578b\u30b3\u30f3\u30b8\u30a7\u30ea\u30f3ConAnc, \u751f\u7269\u7269\u7406, 55, 117 (2015)<\/a><\/p>\n\n\n\n

\u300c\u8010\u71b1\u6027\u30df\u30b9\u30de\u30c3\u30c1\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306e\u5229\u7528\u65b9\u6cd5\u300d, \u767a\u660e\u8005: \u4e0a\u68ee\u9686\u53f8, \u77f3\u91ce\u826f\u7d14, \u76f8\u826f\u6b66\u5b8f, \u77f3\u91ce\u5712\u5b50, \u5c71\u4e0a\u3000\u5065, \u767d\u4e95\u3000\u525b, \u7279\u98582014-184934<\/a> \u5e73\u621026\u5e749\u670811\u65e5, PCT\u51fa\u9858\u756a\u53f7: PCT\/JP2015\/075603(\u5e73\u621027\u5e749\u67089\u65e5) <\/a><\/p>\n\n\n\n

2014<\/p>\n\n\n\n

Ohtana Y, Azamimi A, Altaf-Ul-Amin M, Huang M, Ono N, Sato T, Sugiura T, Horai H, Nakamura Y, Morita A, Lange KW, Kibinge NK, Katsuragi T, Shirai T, <\/em>Kanaya S, Clustering of 3D structure similarity based network of secondary metabolites to reveal their relationships with biological activities, Mol. Inform., 33, 790-801 (2014)<\/a> (Molecular Informatics Best Paper Award 2014)<\/a><\/p>\n\n\n\n

Oda H, Nakagawa K, Ade J, Awaya T, Funabiki M, Hijikata A, Nishikomori R, Funatsuka M, Oshima Y, Sugawara Y, Yasumi T, Kato H, Shirai T, Ohara O, Fujita T, Heike T, Aicardi-Gouti\u00e8res Syndrome Is Caused by IFIH1 Mutations, Am. J. Hum. Genet. 95, 121-125 (2014)<\/a> <\/p>\n\n\n\n

Shirai T*, Saito M, Kobayashi A, Asano M, Hizume M, Ikeda S, Teruya K, Morita M, Kitamoto T, Evaluating prion models on comprehensive mutation data of mouse PrP, Structure, 22, 560-571 (2014)<\/a><\/p>\n\n\n\n

Ogawa T, Shirai T*. Tracing Ancestral Specificity of Lectins: Ancestral Sequence Reconstruction Method as a New Approach in Protein Engineering, In Lectins: Methods and Protocols, Methods in Molecular Biology (ed. J Hirabayashi ) vol. 1200, Springer, pp.539-551 (2014) <\/a><\/p>\n\n\n\n

\u8fbb\u3000\u654f\u4e4b, \u767d\u4e95\u3000\u525b*, \u30d3\u30c3\u30b0\u30c7\u30fc\u30bf\u304b\u3089\u306e\u5c55\u958b:\u53e4\u4ee3\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u3068\u8d85\u5206\u5b50\u30e2\u30c7\u30ea\u30f3\u30b0, \u751f\u547d\u306e\u30d3\u30c3\u30b0\u30c7\u30fc\u30bf\u5229\u7528\u306e\u6700\u524d\u7dda (\u690d\u7530\u5145\u7f8e\u3000\u76e3\u4fee),\u3000\u30b7\u30fc\u30a8\u30e0\u30b7\u30fc\u51fa\u7248, pp. 225-231 (2014)<\/a> <\/p>\n\n\n\n

\u300c\u9ad8\u901f\u30b0\u30e9\u30d5\u30de\u30c3\u30c1\u691c\u7d22\u88c5\u7f6e\u53ca\u3073\u65b9\u6cd5\u300d, \u767a\u660e\u8005: \u767d\u4e95\u3000\u525b, \u7279\u8a31\u7b2c5484946\u53f7\u3000\u5e73\u621026 \u5e742\u670828\u65e5\u3000(\u51fa\u9858\u756a\u53f7\uff1a\u7279\u98582010-31526<\/a> \u5e73\u621022\u5e742\u670816\u65e5), PCT\u51fa\u9858\u756a\u53f7: PCT\/JP2011\/053280<\/a>(\u5e73\u621023\u5e742\u670816\u65e5)<\/p>\n\n\n\n

2013<\/p>\n\n\n\n

Nakae S, Ito S, Higa M, Senoura T, Wasaki J, Hijikata A, Shionyu M, Ito S, Shirai T*, Structure of novel enzyme in mannan biodegradation process 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP, J. Mol. Biol. 425, 4468-4478 (2013)<\/a> <\/p>\n\n\n\n

Sakuma M, Imada K, Okumura Y, Uchiya KI, Yamashita N, Ogawa K, Hijikata A, Shirai T, Homma M, Nikai T, X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus. J. Biol. Chem. 288, 17451-17459 (2013) <\/a><\/p>\n\n\n\n

Ogawa T, Shirai T, Experimental molecular archeology: reconstruction of ancestral mutants and evolutionary history of proteins as a new approach in protein engineering, In Protein Engineering technology and application (ed. T. Ogawa), InTech, pp. 111-132 (2013) <\/a><\/p>\n\n\n\n

\u767d\u4e95\u3000\u525b*, \u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u89e3\u6790\u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u30ea\u30ac\u30f3\u30c9\u306e\u591a\u69d8\u6027, \u751f\u5316\u5b66, 85, 671-687 (2013)<\/a><\/p>\n\n\n\n

2012<\/p>\n\n\n\n

Saito M, Takemura N, Shirai T*, Classification of ligand molecules in PDB with fast heuristics graph match algorithm COMPLIG. J. Mol. Biol. 424, 379-390 (2012)<\/a><\/p>\n\n\n\n

\u767d\u4e95\u3000\u525b*, \u8d85\u5206\u5b50\u30e2\u30c7\u30ea\u30f3\u30b0:DNA\u306e\u8907\u88fd\u904e\u7a0b\u306b\u304a\u3051\u308b\u8907\u88fd\u30e2\u30fc\u30c9\u3068\u6821\u6b63\u30e2\u30fc\u30c9\u306e\u30b9\u30a4\u30c3\u30c1\u30f3\u30b0\u30e1\u30ab\u30cb\u30ba\u30e0. \u751f\u7269\u7269\u7406, 52, 246-249 (2012)<\/a><\/p>\n\n\n\n

\u767d\u4e95\u3000\u525b*,\u7956\u5148\u907a\u4f1d\u5b50\u63a8\u5b9a\u306b\u3088\u308b\u53e4\u4ee3\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u89e3\u6790. \u751f\u7269\u5de5\u5b66, 12, 11-14 (2012)<\/a><\/p>\n\n\n\n

2011<\/p>\n\n\n\n

Oyama T, Ishino S, Fujino S, Ogino H, Shirai T, Mayanagi K, Saito M, Nakagawa N, Ishino Y, Morikawa K, Architecture of archaeal GINS complexes essential for initiation of DNA replication by X-ray crystallography and bioinformatics. BMC Biol. 9:28 (2011) <\/a><\/p>\n\n\n\n

Konno A, Kitagawa A, Watanabe M, Ogawa T, Shirai T *, Tracing protein evolution with ancestral structures of fish galectin. Structure, 19, 711-722 (2011) <\/a>(Faculty of 1000 Recommended paper)<\/p>\n\n\n\n

Shionyu-Mitsuyama C, Waku T, Shiraki T, Oyama T, Shirai T *, Morikawa K, Detecting structural similarity of ligand interactions in the lipid metabolic system of nuclear receptors, lipid binding proteins, and metabolic enzymes. Protein Eng. Des. Sel. 24, 397-403 (2011) <\/a><\/p>\n\n\n\n

Mayanagi K, Nishida H, Kiyonari S, Saito M, Kohda D, Ishino Y, Shirai T *, Morikawa K, The architecture of the DNA polymerase-PCNA-DNA ternary complex. Proc Natl Acad Sci U.S.A. 108, 1845-1849 (2011) <\/a><\/p>\n\n\n\n

Motono C, Nakata J, Koike R, Shimizu K, Shirota M, Amemiya T, Tomii K, Nagano N, Sakaya N, Misoo K, Sato M, Kidera A, Hiroaki H, Shirai T, Kinoshita K, Noguchi T, Ota M, SAHG, a comprehensive database of predicted structures of all human proteins. Nucleic Acid Res. 39, 487-493 (2011) <\/a><\/p>\n\n\n\n

2010<\/p>\n\n\n\n

Konno A, Yonemaru S, Kitagawa A, Muramoto K, Shirai T, Ogawa T, Protein engineering of conger eel galectins by tracing of molecular evolution using probable ancestral mutants. BMC Evol. Biol., 10:43 (2010) <\/a><\/p>\n\n\n\n

Konno A, Ogawa T, Shirai T*, Ancestral protein reconstruction as a new field in protein engineering. In Ribosomal Proteins and Protein Engineering: Design, Selection and Applications (eds. V. Ortendhal and H. Salchow), Nova Science Publishers Inc., pp. 65-90 (2010)<\/a><\/p>\n\n\n\n

\u771f\u67f3\u6d69\u592a, \u5ee3\u660e\u79c0\u4e00, \u767d\u4e95\u3000\u525b, \u69cb\u9020\u6c7a\u5b9a\u6cd5, \u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7acb\u4f53\u69cb\u9020\u5165\u9580\u2013\u57fa\u790e\u304b\u3089\u69cb\u9020\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u3078- (\u85e4 \u535a\u5e78\u7de8), \u8b1b\u8ac7\u793e, pp. 147-178 (2010)<\/a> <\/p>\n\n\n\n

Contributions (2010~)<\/h5>\n\n\n\n

\u65e5\u672c\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u5b66\u4f1a\u3000\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u6280\u8853\u8005\u8a8d\u5b9a\u8a66\u9a13\u5b9f\u884c\u59d4\u54e1\u30002010\u5e74-\u73fe\u5728<\/p>\n\n\n\n

\u65e5\u672c\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u5b66\u4f1a\u3000\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u6280\u8853\u8005\u8a8d\u5b9a\u8a66\u9a13\u5b9f\u884c\u59d4\u54e1\u9577\u30002011\u5e74\u30fb2013-2014\u5e74<\/p>\n\n\n\n

\u95a2\u897f\u30d0\u30a4\u30aa\u30e1\u30c7\u30a3\u30ab\u30eb\u7814\u7a76\u4f1a\u3000\u59d4\u54e1\u9577\u30002011-2014\u5e74\u3000<\/p>\n\n\n\n

\u65e5\u672c\u539f\u5b50\u529b\u7814\u7a76\u958b\u767a\u6a5f\u69cb\u3000\u7814\u7a76\u696d\u7e3e\u8a55\u4fa1\u59d4\u54e1\u30002009\u5e74\u30fb2014\u5e74<\/p>\n\n\n\n

\u5bcc\u5c71\u770c\u7acb\u5927\u5b66\u751f\u7269\u5de5\u5b66\u79d1\u3000\u975e\u5e38\u52e4\u8b1b\u5e2b(\u30d0\u30a4\u30aa\u60c5\u5831\u5b66)\u30002012\u5e74-\u73fe\u5728<\/p>\n\n\n\n

\u65e5\u672c\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u5b66\u4f1a\u3000\u8a55\u8b70\u54e1\u30fb\u5e79\u4e8b\u30fb\u4f1a\u9577\u88dc\u4f50\u30002014-2015 \u8a55\u8b70\u54e1\u30fb\u5e79\u4e8b 2016-\u73fe\u5728<\/p>\n\n\n\n

\u6771\u4eac\u5927\u5b66\u8fb2\u5b66\u7814\u7a76\u79d1\u3000\u975e\u5e38\u52e4\u8b1b\u5e2b(\u60c5\u5831\u751f\u547d\u5de5\u5b66 ) 2015\u30fb2017\u5e74<\/p>\n\n\n\n

\u4eac\u90fd\u5927\u5b66\u85ac\u5b66\u7814\u7a76\u79d1\u3000\u975e\u5e38\u52e4\u8b1b\u5e2b(\u30cf\u3099\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u7406\u8ad6) 2016\u30fb2019<\/p>\n\n\n\n

AMED\u3000\u79d1\u5b66\u6280\u8853\u8abf\u67fb\u54e1\u30002016\u5e74-\u73fe\u5728<\/p>\n\n\n\n

\u6ecb\u8cc0\u5927\u5b66\u30c7\u30fc\u30bf\u30b5\u30a4\u30a8\u30f3\u30b9\u5b66\u90e8\u3000\u7279\u5225\u62db\u8058\u6559\u6388 2016\u5e74-\u73fe\u5728<\/p>\n\n\n\n

\u7b51\u6ce2\u5927\u5b66\u8a08\u7b97\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u3000\u904b\u55b6\u5354\u8b70\u4f1a\u59d4\u54e1 2018\u5e74-\u73fe\u5728<\/p>\n","protected":false},"excerpt":{"rendered":"

Research grants (2010~) \u57fa\u76e4\u7814\u7a76(A)\u3000\u300c\u65b0\u898f\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u958b\u767a\u3092\u898b\u636e\u3048\u305f\u65b0\u898fCas\u30bf\u30f3\u30d1\u30af\u8cea\u5019\u88dc\u306e\u6a5f\u80fd\u89e3\u6790\u300d\u5206\u62c5 2020-2022\u5e74(\u4e88\u5b9a) \u57fa\u76e4\u7814\u7a76(B)\u3000\u300c\u5929\u7136\u5909\u6027\u30d2\u30b9\u30c8\u30f3\u69d8\u86cb\u767d\u8cea\u306b\u3088\u308b\u3001\u7d50 … <\/p>\n

“\u696d\u7e3e(Results)” \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":2,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=\/wp\/v2\/pages\/3","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3"}],"version-history":[{"count":36,"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=\/wp\/v2\/pages\/3\/revisions"}],"predecessor-version":[{"id":276,"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=\/wp\/v2\/pages\/3\/revisions\/276"}],"up":[{"embeddable":true,"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=\/wp\/v2\/pages\/2"}],"wp:attachment":[{"href":"https:\/\/sird.nagahama-i-bio.ac.jp\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}